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. 2017 Jul 28;37(16):e00001-17. doi: 10.1128/MCB.00001-17

FIG 3.

FIG 3

Regulation of EC angiogenic activities by let-7 target HMGA2 gene. (A) Representative Western blot analysis showing regulation of HMGA2 protein level by let-7 silencing or overexpression in HUVEC cells. Tubulin was used as a loading control. The band density is indicated. (B) Silencing of HMGA2 by specific siRNAs in ECs, as demonstrated by Western blotting. Tubulin was used as a loading control. (C) EC migration in response to VEGF treatment after HMGA2 knockdown with siRNAs. (D) Quantification of migration distance (in micrometers). ***, P < 0.001. (E) Quantification of VEGF-induced proliferation indicated by BrdU incorporation after HMGA2 silencing in HUVECs. ***, P < 0.001. (F) Representative pictures of in vitro Matrigel assays after HMGA2 silencing in HUVECs. (G) Quantification of branching point per field in panel F. ***, P < 0.001.