FIG 4.

3K acetylation of cavin-1 recruits HSL to the plasma membrane. (A) Cavin-1 localizes in the plasma membrane of 3T3-L1 adipocytes. The subcellular localization of endogenous cavin-1 in 3T3-L1 adipocytes was examined by immunofluorescence using anti-cavin-1 antibodies. The nuclei were stained with DAPI. DIC, differential interference contrast. (B) 3K acetylation of cavin-1 recruits HSL to the plasma membrane. 3T3-L1 adipocytes were infected with recombinant adenovirus expressing Myc-tagged cavin-1^WT, cavin-1^3R, and cavin-1^3Q. The subcellular localization of Myc-cavin-1 (red) and endogenous HSL (green) was examined by immunofluorescence using anti-Myc and anti-HSL antibodies.