FIG 7.

3K acetylation of cavin-1 is under nutritional regulation. (A and B) Glucose and fatty acids increase cavin-1 acetylation in 3T3-L1 adipocytes. Cells were incubated for 3 h in either low-glucose (5 mM) or high-glucose (25 mM) medium (A) or with 500 μM oleic acid (OA) (B) and subjected to Western blotting with the indicated antibodies. (C and D) 3K acetylation of cavin-1 in white adipose tissue is regulated by nutritional status. (C) C57BL/6 male mice at 20 weeks of age were fasted for 48 h. (D) C57BL/6 male mice at 4 weeks of age were fed with either a normal chow diet (NCD) or a high-fat diet (HFD) for 16 weeks. (E) A model for 3K acetylation of cavin-1 in regulating lipolysis as a reversible posttranslational modification in adipocytes. Cavin-1 acetylation promotes lipolysis by docking HSL at the surface of the caveolae in the plasma membrane.