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Survival of ER stress by activation loop mutants requires D836. Survival of ER stress induced with 0.8 μg/ml Tm or 1.5 mM DTT. Serial 10-fold dilutions of fresh overnight cultures of ire1Δ cells expressing the indicated IRE1 alleles from YCplac33 or carrying the empty vector were spotted on SD-minus-uracil plates containing 0.8 μg/ml Tm or 1.5 mM DTT and allowed to grow for 2 to 3 days before taking photographs. The experiment was repeated three times with qualitatively similar results.
