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. 2017 Jul 28;37(16):e00655-16. doi: 10.1128/MCB.00655-16

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Copyright © 2017 Armstrong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 6 — Survival of ER stress by activation loop mutants. Survival of ER stress induced with 0.4 and 0.8 μg/ml tunicamycin (Tm). Serial 10-fold dilutions of fresh overnight cultures of ire1Δ cells expressing the indicated IRE1 alleles from YCplac33 or carrying the empty vector (−) were spotted on SD minus uracil plates containing 0.4 or 0.8 μg/ml Tm and allowed to grow for 2 to 3 days before taking photographs. Rows labeled #1 and #2 indicate two independent transformants for the S840A S841A and Q-A mutants. The experiment was repeated three times with qualitatively similar results.