FIG 5.
The chaperon effect of VP35 on NP. (A) SDS-PAGE analysis of MARV NPcore (left lines) and NPcore-VP35 peptide complex (right lines) self-degradation. Each line contains the same quantity of original proteins. Time is given in hours (h) or days (d). The speculated region of MARV NPcore is boxed in red. Experiments were conducted in three individual repeats. (B) EMSA of the affinity competitions of VP35 peptide and ssRNA for NP. NP was pretreated with ssRNA and then incubated with VP35 peptide or in reverse order before sample loading. The dosages (final concentrations) are listed on the top. The free and combined ssRNAs are labeled on the side. Experiments were repeated more than three times. (C) Electrostatic surface potential (calcluated with APBS tools, with limits of ±5 kbT/ec) and cartoon representation for structure comparison of P/VP35 peptide or Ni-1 arm effect on NP (N). The P (green)/VP35 (pink) peptides, Ni-1 arms (orange), and α20 helices (cyan) are shown as a cartoon and labeled.