TABLE 2.

Permeabilization of synthetic bilayers by variants of the EBOV and RESTV delta peptides

Bilayera	LIC50b											MelP5c
	EBOV peptides							RESTV peptides
	E40ox	E40red	E23ox	E23red	E17ox	E15ox	E14ox	R42ox	R42red	R25ox	R25red
PC vesicles	>50	>50	16	>50	>50	>50	>50	I	I	>50	I	0.3
PC bilayerd	ND	ND	0.5	I	ND	ND	ND	ND	ND	0.6	I	0.1
30% PG vesicles	6	18	3	8	ND	ND	ND	22	>50	>50	>50	1.6
50% PG vesicles	2.3	6.1	2.9	10	8.6	15	48	ND	ND	ND	ND	1.2

^aPC vesicles and bilayers are made from 1-palmitoyl-2-oleoyl-phosphatidylcholine (POPC). PC/PG bilayers also contain 30% or 50% 1-palmitoyl-2-oleoyl-phosphatidylglycerol (POPG).

^bLIC₅₀ is the P/L ratio required to induce 50% leakage of the dyes ANTS and DPX from lipid vesicles or 50% loss of bilayer resistance in a polymer-cushioned supported lipid bilayer. The LIC₅₀ values in the table are multiplied by 1,000, i.e., 1 is equivalent to an LIC₅₀ (P/L ratio) of 1:1,000. >50, more than 5% leakage was observed at the highest concentration but the LIC₅₀ is greater than 0.05; I (inactive), less than 5% permeabilization was observed at the highest concentration (Fig. 5). Oxidized peptides (ox) have a disulfide cross-link between the conserved cysteines, while reduced peptides (red) do not. All values are the mean of ≥3 experiments. Standard deviations were 20% to 25% of the values listed.

^cMelP5 is a lytic control peptide derived from the bee venom toxin, melittin (23).

^dPolymer-cushioned, surface-supported PC bilayers on silicon (see the text).