Lake Sinai virus variants identified in this study. (A) Genome structures of LSV strains identified in The Netherlands (GenBank accession number KY354242), Tonga (accession number KY354241), and South Africa (accession numbers KY354243 to KY354244), compared to previously characterized genomes of LSV-1 and LSV-2. Open reading frames are blue, and conserved functional domains are indicated (NCBI protein sequence accession numbers ARO50053 to ARO50067). (B) Maximum likelihood phylogenetic tree of nucleotide alignment of LSV strains from The Netherlands, Tonga, and South Africa with LSV-1 and -2 and other strains described previously (26).