FIG 2.

Spread of the wild-type and the SAT⁻ mutant viruses in PT cells at a low multiplicity of infection (MOI, 0.01). Infected cells (red) were visualized with the assembled capsid-specific 3C9 primary antibody and the CF594 secondary antibody. (A) Cells were fixed at the indicated time points, and their nuclei were labeled with Hoechst 33342. (B) 3C9 antibody was added to Kresse-infected cells to monitor the inhibition of the secondary infections. The times of the beginning of the treatments are indicated. The cells were fixed at 24 h p.i.