FIG 2.

Inhibition of innate immune responses triggered by 2′,3′-cGAMP or the ΔICP0 virus in cells expressing the HSV-1 UL46 protein. (A) HEL cells constitutively expressing the Myc-UL46 protein. (B) HEL or HEL-UL46 cells were either treated with 2′,3′-cGAMP (3 or 10 μM) (lanes 4, 5, 10, and 11), exposed to the ΔICP0 virus (1 or 5 PFU/cell) (lanes 6, 7, 12, and 13), or left untreated (lanes 3 and 9). At 8 h posttreatment, the cells were harvested and the ISG56 transcripts were semiquantified. 18S rRNA served as a control. (C) HEL or HEL-UL46 cells were treated with 2′,3′-cGAMP or exposed to the ΔICP0 virus, similar to the description for panel B. The ISG56, IL-6, ISG15, IL-1β, and TNF-α transcripts were quantified in duplicate assays by real-time PCR analysis relative to their transcripts in untreated HEL cells, indicated by the arrow. 18S rRNA was used for normalization. Each experiment was repeated three times. Results of a representative experiment are depicted.