FIG 4.

Elimination of STING and IFI16 in cells expressing UL46. (A) HEL, HEL-UL46, HEp-2, and HEp-UL46 cells were either treated with 2′,3′-cGAMP (3 μM) or left untreated. The cells were harvested at 8 h after the addition of 2′,3′-cGAMP, and protein analysis was done with antibodies against STING, IFI16, and β-actin. (B) HEK-293 cells were either mock transfected or transfected with the pcDNA 3.1 Myc-UL46 or with the pEGFP-N3 plasmid. The cells were harvested at 72 h after transfection, and immunoblotting was done with the STING, IFI16, and β-actin antibodies. (C and D) Total RNA was extracted from replicate cultures of cells described for panels A and B, and the STING and IFI16 transcripts were semiquantified by PCR. 18S rRNA served as a loading control.