FIG 3.

Impact of GuHCl treatment on full-length and terminally deleted luciferase replicon kinetics. Full-length (A and B) and deleted (C and D) luciferase replicon transcripts described in the legend to Fig. 2 were transfected into human (HCM) (A and C) and murine (HL-1) (B and D) cardiac myocyte monolayers in the presence or absence of GuHCl (an inhibitor of enterovirus RNA replication) at a final concentration of 3 mM. At 2, 4, 6, and 8 h posttransfection, cells were harvested and luciferase activity was measured. The y axis shows log₁₀ units of luciferase activity. Luciferase units in the presence and absence of GuHCl are shown in each graph. Although not readily visible, standard deviations (as error bars) are given for each data point. The results presented are the products of three independent experiments.