FIG 1.

MeRIP–qRT-PCR measurement of m⁶A-mRNA and total mRNA of KSHV transcripts. (A) Schematic presentation of MeRIP procedure. (B) Melting temperature (T_m) of qRT-PCR product of KSHV ORF50 (RTA). Positive signals were seen only with cDNAs from the input and the product of RIP with anti-m⁶A (RIP-m⁶A). No signal was seen with cDNAs from the product of RIP with control IgG (RIP-IgG). (C and D) Percentages of m⁶A-mRNA of β-actin in the products of RIP with m⁶A (C) and IgG (D), noting that the levels of m⁶A-mRNA of β-actin from BCBL1 cells treated with PBS or TPA for 24 h were several hundred times higher in the products of RIP with m⁶A than in the products of RIP with IgG.