FIG 14.

m⁶A modification of site G in ORF50 (RTA) pre-mRNA is required for recruitment of YTHDC1 and SRSF3 and disassociation of SRSF10. (A) Location of the m⁶A site G region analyzed by RIP–qRT-PCR with antibodies to YTHDC1, SRSF3, and SRSF10 and the specific primers listed in Table 2. (B) Relative levels of RNA bound by YTHDC1, SRSF3, or SRSF10 and the levels of m⁶A in the site G region in BCBL1 cells treated with PBS or TPA for 24 h. For all comparisons, the levels of RNA and m⁶A in cells treated with PBS were set equal to 1. (C) Relative levels of RNA bound by YTHDC1, SRSF3, or SRSF10 and levels of m⁶A in the site G region in 293T cells transfected with wild type (WT) pExon1-intron-exon2-GFP or its mutant with site G mutated (Mut-G). For all comparisons, the levels of RNA and m⁶A in cells transfected with the wild-type plasmid were set equal to 1. *, differences with a P value of <0.05.