FIG 2.

The levels of total mRNA and m⁶A-mRNA of KSHV lytic transcripts increase in parallel when cells are stimulated for lytic replication. (A) Levels of total mRNA and m⁶A-mRNA of the 5.8-kb and 5.40-kb tricistronic latent transcripts encoding LANA (ORF73), viral cyclin (ORF72), and viral FLIP (ORF71) and the 1.7-kb bicistronic latent transcript encoding viral cyclin (ORF72) and viral FLIP (ORF71) in BCBL1 cells treated with PBS (placebo control) or TPA (20 ng/ml) for 24 h. (B) Levels of total mRNA and m⁶A-mRNA of lytic transcripts ORF45, ORF49, ORF50 (RTA), ORF57, ORF59, and ORFK8 from the cells described in the legend to panel A. All qRT-PCRs were conducted in triplicate. The statistical significance of the differences in the level of m⁶A-mRNA or total mRNA of a given transcript between cells treated with PBS and cells treated with TPA was analyzed by an unpaired t test. *, differences with P values of <0.05 (n = 3).