FIG 4.

Posttranscriptional m⁶A modification of KSHV transcripts also occurs in endothelial cells and can be induced by different lytic replication stimuli. (A) Levels of total mRNA and m⁶A-mRNA of KSHV lytic transcripts ORF50 (RTA) and ORF57 in TIVE-KSHV cells treated with PBS or TPA for 24 h. (B) Levels of total mRNA and m⁶A-mRNA of ORF50 (RTA) in BCBL1 cells treated with PBS (placebo), TPA (20 ng/ml), H₂O₂ (400 μM), NaB (0.5 mM), or TNF-α (10 ng/ml) for 24 h. The statistical significance of the differences in the level of m⁶A-mRNA or total mRNA of a given transcript between cells treated with PBS and cells treated with different stimuli was analyzed by an unpaired t test. *, differences with P values of <0.05 (n = 3).