TABLE 1.

PCR primer sequences used

ZIKV PCR target(s)	Primer sequence (5′→3′)
Capsid	Forward: ATATATGCTAGCGTTTAAACGCCACCATGAAAAACCCAAAAAAGAAATCC
prM	Forward: ATATATGCTAGCGTTTAAACGCCACCATGGGCGCAGATACTAGTGTCGGAAT
Envelope	Forward: ATATATGCTAGCGTTTAAACGCCACCATGGGAAGCTCAACGAGCCAAAAAGTC
NS2A	Forward: ATATATGCTAGCGTTTAAACGCCACCATGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGTAACTTAGTAAGGTCAAT
NS2B	Forward: ATATATGCTAGCGTTTAAACGCCACCATGAGCTGGCCCCCTAGCGAAGTACTC
NS3	Forward: ATGCCACCTTCACTTCACGTCTAC
NS2B-3	Forward: ATATATGCTAGCGTTTAAACGCCACCATGAGCTGGCCCCCTAGCGAAGTACTC
NS4A	Forward: ATATATGCTAGCGTTTAAACGCCACCATGGGAGCGGCTTTTGGAGTGATGGAA
NS4B	Forward: ATATATGCTAGCGTTTAAACGCCACCATGTCTCCCCAGGACAACCAAATGGCA
NS5	Forward: GAGGAGAGTGCCAGAGTTGTGTGT
All 3× FLAG targets	Reverse: GGCGGGAGCGGCGGGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTAGGGCGCGCCCTCGAGATATAT
myc-DENV capsid	Forward: ATTAGCGCTAGCATGGAACAAAAACTCATCTCAGAAGAGGATCTGAATGACCAACGGAAAAAGGC
	Reverse: GTACGGATCCTTATCTGCGTCTCCTATTCAAGA
myc-JEV capsid	Forward: ATTAGCGCTAGCATGGAACAAAAACTCATCTCAGAAGAGGATCTGACTAAAAAACCAGGAGGGC
	Reverse: GTACGGATCCTTATCTTTTGTTTTGCTTTCTGCC
myc-MVEV capsid	Forward: ATTAGCGCTAGCATGGAACAAAAACTCATCTCAGAAGAGGATCTGTCTAAAAAACCAGGAGGAC
	Reverse: GTACGGATCCTTATCTTTTCTTTTGTTTTTTGCC
myc-WNV capsid	Forward: ATTAGCGCTAGCATGGAACAAAAACTCATCTCAGAAGAGGATCTGTCTAAGAAACCAGGAGGGCC
	Reverse: GATCGGATCCTTATCTTTTCTTTTGTTTTGAGC
myc-YFV capsid	Forward: ATTAGCGCTAGCATGGAACAAAAACTCATCTCAGAAGAGGATCTGTCTGGTCGTAAAGCTCAGGG
	Reverse: GTACGGATCCTTATTAACGGCGTTTCCTTGAG