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. 2017 Jul 27;91(16):e00584-17. doi: 10.1128/JVI.00584-17

FIG 1.

FIG 1

IBDV infection upregulates the expression of VDAC1. (A) IBDV infection causes upregulation of VDAC1 expression in DF-1 cells. DF-1 cells were mock infected or infected with IBDV at an MOI of 1. Cells were harvested at 12, 24, and 36 h postinfection (p.i.) and then analyzed by Western blotting with anti-VDAC1 and -VP3 antibodies. (B) Relative intensities of VDAC1 in DF-1 cells. β-Actin was used as a protein loading control. Representative results are shown, with bars representing the intensities of VDAC1/actin normalized to the control condition. (C) IBDV infection causes upregulation of VDAC1 mRNA expression in DF-1 cells. DF-1 cells were mock infected or infected with IBDV at an MOI of 1. Cells were harvested at 24 and 36 h postinfection and then analyzed by qRT-PCR. (D) IBDV infection causes upregulation of VDAC1 expression in 293T cells. 293T cells were infected and analyzed as described for panel A. (E) Relative intensities of VDAC1 in 293T cells. β-Actin was used as a protein loading control. Representative results are shown, with bars representing the intensities of VDAC1/actin normalized to the control condition. Data are means and SD for three independent experiments. *, P < 0.05; **, P < 0.01; ****, P < 0.0001 versus control (unpaired t test).