FIG 4.
Pathogenesis in pigs infected with chimeric FMDVs. Pigs (n = 2) were challenged with the virus (105.0 TCID50/0.1 ml) intradermally in each footpad. (A) Clinical scores of chimeric FMDV-challenged pigs over time. All pigs in the experiment were challenged with recombinant FMDV (105.0 TCID50/0.1 ml) in each footpad. (B) Detection of virus in nasal discharge by real-time RT-PCR (rRT-PCR). (C) Detection of virus in sera by real-time RT-PCR. (D) Comparison of clinical scores and detection of virus in sera and nasal swabs. Shown are total clinical scores throughout the experimental period, total viremia throughout the experimental period, and total virus counts in nasal discharge during the experimental period in inoculated pigs. (E) Antibody responses (virus-neutralizing titers [left axis] and percent inhibition ratios in an NSP ELISA [right axis]) to the original wild-type serotypes and chimeric FMDV in pigs after chimeric FMDV challenge. The cutoff for a positive reaction in the NSP ELISA is >50% inhibition.