TABLE 3.

Primers used to replace the P1 genes of seven serotypes in pO Mamisa-FG

Serotype or strain	Forward primer	Target region for forward primer	Reverse primer	Target region for reverse primer
O Manisa	5′-CTTCTAAATTTTGACCTGCTCAAAT-3′	2A	5′-CTTGAGCCTTTTCTGGACCTTTGT-3′	L
O-PanAsia2	5′-GGCGCCGGGCAATCCAGCCCGGCG-3′	VP4	5′-CTGTTTCACAGGTGCCACTATCT-3′	VP1
A22	5′-GGAGCCGGGCAATCCAGTCCGGCG-3′	VP4	5′-TTGTTTTGCAGGTGCAATGATCTTC-3′	VP1
AsMOG	5′-GGAGCCGGGCAATCCAGTCCGGCG-3′	VP4	5′-CTGTTTCTCAGGTGCAATGATCTCC-3′	VP1
C-Ob	5′-GGAGCTGGGCAATCCAGCCCAGCG-3′	VP4	5′-TTGTTTTGCAGGTGCGACGAGCGG-3′	VP1
SAT1-SA	5′-GGAGCAGGTCAGTCGTCACCAGCT-3′	VP4	5′-CTGCTTGGCAGGTTTGACGAGGGC-3′	VP1
SAT2-SAU	5′-GGAGCCGGGCAATCCAGCCCAGCC-3′	VP4	5′-CTGCCTCTCGACGCCAATGGGCGC-3′	VP1
SAT3-SA	5′-GGAGCGGGTCAGTCGTCCCCTGCC-3′	VP4	5′-TTGTTTGTCAGGTGCCACCAGTTTG-3′	VP1