FIG 3.

Analysis of HTLV-1 Gag expression in cells and packaged into virus particles. (A) ELISA. HTLV-1 MA (p19) production from MT-2 and SP cells was determined by using a p19-based ELISA as described in Materials and Methods. (B) Immunoblot analysis. HTLV-1 proteins from cell lysates and released into cell culture supernatants of MT-2 and SP cells were analyzed by immunoblot analysis at day 0 or day 10 posttransfection (with a 1:2 split at day 6) as described in Materials and Methods. γ-tub, gamma tubulin. Shown are representative data from 3 independent experiments.