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Characterization of recombinant viruses with F117S and HN1169R mutations. Recombinant viruses that contained either F117S or HN169R or both were used to infect HT1080 cells or HeLa cells. (A) Bright-field images were acquired with an EVOS microscope at 14 h p.i. in cells infected at an MOI of 1. (B) Cell killing was measured using a CellTiter-Glo assay on day 2 postinfection at an MOI of 0.1. ns, not significant.
