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. 2017 Jul 27;91(16):e00823-17. doi: 10.1128/JVI.00823-17

FIG 1.

FIG 1

SVV infection does not trigger host type I interferon production. (A) Luciferase activity assay from 293T cells infected with Sev (HA titer, 32) or SVV (MOI, 1) for the indicated times. (B) qRT-PCR assay performed with 293T cells infected with Sev (HA titer, 32) and SVV (MOI, 1) for the indicated times. (C) Immunoblot analysis for IRF3 activation in 293T cells infected with Sev (HA titer, 32) or SVV (MOI, 1) for the indicated times. Quantification of the extent of the increase in phosphorylated IRF3 was normalized to total IRF3 (right panel). (D) Immunofluorescence microscopy analysis for the nuclear translocation of IRF3 in 293T cells infected with Sev (HA titer, 32) or SVV (MOI, 1) for 12 h. IRF3 is green and the nucleus is blue. (E) 293T cells were pretreated with IFN-β at the indicated concentration for 12 h and then infected with VSV-green fluorescent protein (GFP) or SVV. CPE was observed at 24 h postinfection. (F) The virus progeny from panel E were determined by plaque assay. Data are shown as means ± SD. ***, P < 0.001. Data are representative of those from at least three independent experiments.