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SVV 3Cpro interacts with MAVS, TRIF, and TANK. 293T cells were transfected with Flag-MAVS and empty vector, HA-3Cwt, or HA-3Cdm for 24 h. Subsequently, the interaction between MAVS and 3Cpro was measured by anti-Flag immunoprecipitation (A). (B) Anti-Flag immunoprecipitation for the interaction between Flag-TRIF, HA-3Cwt, and HA-3Cdm. (C) Anti-Flag immunoprecipitation for the interaction between Flag-TANK, HA-3Cwt, and HA-3Cdm. WCL, whole-cell lysate.
