FIG 6.
SVV 3Cpro cleaves MAVS, TRIF, and TANK at specific sites. (A) SVV polyprotein organization and the proposed SVV 3Cpro cleavage sites (red). The indicated viral proteins were colored. (B) (Top) Schematic representation of Flag-MAVS and its mutants. (Bottom) 293T cells transfected with the indicated Flag-MAVS construct and empty vector or HA-3Cwt were lysed and subjected to immunoblot analysis. CARD, caspase recruitment domain; TM, transmembrane. (C) (Top) Schematic representation of Flag-TRIF and its mutants. (Bottom) 293T cells transfected with the indicated Flag-TRIF construct and empty vector or HA-3Cwt for were lysed and subjected to immunoblot analysis. NTD, N-terminal domain; TIR, Toll/interleukin-1 receptor domain; SIA, sufficient to induce apoptosis. (D) (Top) Schematic representation of Flag-TANK and its mutants. (Bottom) 293T cells transfected with the indicated Flag-TANK construct and empty vector or HA-3Cwt for were lysed and subjected to immunoblot analysis. TBD, TRAF binding domain; ZF, zinc finger.