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. 2017 Mar 13;6(3):e300. doi: 10.1038/oncsis.2017.3

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Silencing FAM83A inhibits pancreatic CSC-like traits in vitro. (a) Western blotting analysis of FAM83A expression in PANC-1 and CFPAC-1 pancreatic cancer cells stably expressing FAM83A shRNAs. (b) Flow cytometry analysis of the CD133⁺ population in the indicated cells. (c) Representative images of the tumorspheres formed by the indicated cells (left). Scale bar: 100 μm. Histograms (right) showing the mean number of spheres formed. (d) Hoechst 33342 dye exclusion assay showing that silencing FAM83A decreased the number of SP⁺ cells. (e) Effects of chemotherapeutic drugs on pancreatic cancer cells. Pancreatic cancer cells were seeded in culture plates and incubated with and without 50 μM gemcitabine and 5-FU for 24 and 72 h. Apoptotic cells were measured by FACS analysis. Each bar represents the mean±s.d. of three independent experiments; *P<0.05.