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Insertion Site Identification and Putative Insertion Contig Structure. Read-1 of each pair generated by hemi-specific PCR is a split read that contains both P-element and adjacent genomic sequence. Breakpoints are determined based on the alignment of read-1 (red) to the plus (a) or minus genomic strand (b). Contigs are constructed through insertion of the P-element consensus at the insertion site, which is flanked by an 8 bp target site duplication on either side
