Table 1.
Reference standard positive ATCC specimens used in the validation of the new primer set, and species identification of positive clinical specimens
| Source | Specimen | Species | Reference standard assay positivea |
|---|---|---|---|
| ATCC | ATCC50372 | A. polyphaga | Both |
| ATCC30173 | A. polyphaga | Both | |
| ATCC50492 | A. polyphaga | Both | |
| ATCC PRA-220 | Acanthamoeba spp. | Both | |
| ATCC50372 | A. polyphaga | Both | |
| ATCC50373 | A. castellanii | Both | |
| ATCC50492 | A. castellanii | Both | |
| ATCC50493 | A. castellanii | Both | |
| ATCC50739 | A. castellanii | Both | |
| ATCC30171 | A. culbertsoni | Qvarnstrom | |
| Clinical specimen | 1 | A. castellanii | Both |
| 2 | A. hatchetti | Qvarnstrom | |
| 3 | A. castellanii | Both | |
| 4 | A. castellanii | Qvarnstrom | |
| 5 | Unknown | Qvarnstrom | |
| 6 | A. polyphaga | Both | |
| 7 | Unknown | Riviere | |
| 8 | A. lenticulata | Riviere | |
| 9 | A. castellani | Riviere | |
| 10 | A. griffini | Riviere | |
| 11 | Unknown | Riviere | |
| 12 | A. castellanii | Both | |
| 13 | A. castellanii | Both | |
| 14 | Unknown | Riviere | |
| 15 | A. lenticulata | Qvarnstrom | |
| 16 | Unknown | Riviere | |
| 17 | A. hatchetti | Both | |
| 18 | A. polyphaga | Both | |
| 19 | Unknown | Qvarnstrom | |
| 20 | Unknown | Qvarnstrom | |
| 21 | A. quina | Qvarnstrom |