Fig. 3.

Knockdown of CCDC109B inhibits proliferation, migration, and invasion of glioma cells in vitro. Knockdown efficiency of CCDC109B in U87MG and U251 cells was determined in a by qRT-PCR and in b by western blot analysis; c EdU assays for U87MG- and U251-NC or sh-CCDC109B-1 cells. Magnification ×200. d Graphic representation of ratios of EdU positive cells in U87MG- and U251-NC and sh-CCDC109B-1 cells. Data are presented as the mean ± SEM. e Representative images of colony forming assays for U87MG- and U251-NC (top) or shCCDC109B-1 cells (bottom). f Graphic representation of colony forming results in U87MG- and U251-NC and -sh-CCDC109B-1. Data are presented as the mean ± SEM. g Images of Transwell migration and invasion assays performed with U87MG- and U251-NC and sh-CCDC109B-1 expressing cells. Magnification ×100. h Graphic representation of cell counts from Transwell assays after a 24 h incubation. Experiments were performed in triplicate and counted from 5 random fields. Data are presented as the mean ± SEM. i Western blot analysis for the expression of MMP2 and MMP9 in NC and sh-CCDC109B-1 U87MG and U251 glioma cell lines (*P < 0.05, **P < 0.01, ***P < 0.001)