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. Author manuscript; available in PMC: 2017 Jul 29.
Published in final edited form as: Exp Biol Med (Maywood). 2011 Nov 23;236(12):1379–1388. doi: 10.1258/ebm.2011.011234

Figure 4.

Figure 4

Homocysteine (Hcy) had no effect on intracellular glutathione (GSH) concentrations in early stage of 3T3-L1 preadipocyte adipogenesis. Two days after plating (D0), 3T3-L1 cells were treated with Hcy (0.1 mmol/L) in the differentiation medium for 24 h and the adherent cells were collected and deproteinized with 10% metaphosphoric acid to determine the intracellular GSH and glutathione disulfide (GSSG) concentration. Hormone stimulation for 24 h elevated intracellular GSH concentrations and GSH/GSSG ratio in 3T3-L1 preadipocytes (a, c); however, intracellular GSSG concentrations were not affected (b). Hcy addition did not change the elevation of hormone-induced increase in intracellular GSH status. Data are means ± SD (n = 3). *P < 0.05 compared with the 3T3-L1 cells on D0