Table 2.
Summary of immunohistochemistry and immunoblot results
| Antibody | Cat number | Signal by IHC | α1 lysate | α2 lysate | α3 lysate | α1 α2 α3 lysate | Figures |
|---|---|---|---|---|---|---|---|
| COL6A1 H200 | sc-20649 | ++ | ++ (R) | − | − | + | 1 |
| COL6A1 | ab199720 | +++ | +++ (R)/+++ (NR) | − | − | + | 1 |
| COL6A1 | ab182744 | ++ | ++ (R) | − | − | + | 1 |
| COL6A1 | 17023-1-AP | +++ | +++ (R)/+++ (NR) | − | − | + | 1 |
| COL6A1 B4 | sc-377143 | − | +++ (R) | − | − | + | 1 |
| COL6A2 | 14853-1-AP | ++ | − | ++ (R)/+ (NR) | − | nd | 2 |
| COL6A2 EPR7889 | ab180855 | +++ | − | +++ (R) | − | − | 2 |
| COL6A2 | ab172606 | ++ | − | + (R) | − | nd | 2 |
| COL6A2 D20 | sc-83607 | +++ | − | +++ (R) | − | ++ | 2 |
| COL6A2 H300 | sc-292186 | ++ | − | + (R) | − | + | 2 |
| COL6A2 K15 | sc-377143 | +++ | − | + (R) | − | + | 2 |
| COL6A2 B7 | sc-374566 | − | − | +++ (R)/+ (NR) | − | ++ | 2 |
| COL6A3 G18 | sc-131139 | ++ | − | − | + (NR) | + | 3 |
| COL6A3 3C4 | MAB1944 | +++ | − | − | + (R)/++ (NR) | − | 3 |
| COL6A3 3C4 | sc-47712 | − | − | + | nd | 3 | |
| COL6A3 H300 | sc-367543 | +++ | − | − | − | − | 3 |
| COL6A3 H3-2 | sc-81766 | − | − | − | − | − | 3 |
| COL6A3 64C11 | ab49273 | − | − | − | − | − | 3 |
| COL6A3 N12 | sc-131140 | − | − | − | − | nd | 3 |
| Collagen VI VI-26 | MAB3303 | +++ | − | − | − | + | 4 |
| Collagen VI | 70R-CR009X | +++ | − | − | ++ (NR) | +++ | 4 |
| Collagen VI 172C2 | sc-47764 | − | − | − | ++ (NR) | − | 4 |
| Collagen VI 5C6 | − | − | − | − | + | 4 | |
| Collagen VI | AB7821 | − | − | Non-sp. | Non-sp. | ++ | 4 |
Each antibody is scored for signal intensity on immunohistochemical signal and, for immunoblots, band intensity ranging from ‘−’ representing no detectable signal, to ‘+++’ representing strong signal. The description of immunoblots signal strength scores reduced (R) and non-reduced (NR) samples separately. Some blots on triple transfected lysates were not conducted and are designated as not determined (nd)