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. 2017 Jun 12;6(7):1–12. doi: 10.1093/gigascience/gix043

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© The Authors 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1: — Organization of the rrn region in bacteria. (A) Hypothetical transcriptional arrangements expected for rrn and tested experimentally using 2 sets of primer pairs (see small arrows drawn in each configuration). (B) Agarose gel electrophoresis of PCR reactions performed under the 2 hypothetical arrangements of rrn; lanes: (i) 1 kb ruler (Fermentas), (ii) PCR reaction from the top configuration in (A), (iii) PCR reaction from the bottom configuration in (A). The GelAnalyser Java application was used to perform the band size analysis of the 1 kb ruler standard (C) and the amplicons obtained from human faecal DNA (D).