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. 2017 Jun 12;6(7):1–12. doi: 10.1093/gigascience/gix043

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© The Authors 2017. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3: — Microbial structure of the mock communities. (A and B) Microbial species and respective relative proportions determined to be present in the HM782D and D6305 mock communities, respectively, following the analysis of raw data obtained from rrn amplicon sequencing in MinION™ and chemistry R9. (C and D) Comparative analysis of the expected microbial species and proportions against the data obtained after mapping reads generated by a 4.5-kbp amplicon PCR and sequenced in the MinION™ device with R9 and R9.4 chemistries, for HM782D and D6305, respectively. (E) Linear correlation analysis of relative read proportions obtained for all bacterial species present in HM872D and D6305 mock communities with R9 and R9.4 chemistries.