Figure 7.

β-catenin expression within MSCs cultures. MSCs were immunostained for β-catenin (green) and nuclear counterstained with DAPI (blue), following a two-step protocol that involved the culturing of cells (1) for 2 days in the absence or presence of BIX01294 and then (2) for an additional 2 days with or without Wnt11. (a) MSCs cultured without treatment contained many brightly stained β-catenin-positive cells. (b) Cultures treated with BIX01294 without Wnt11 or (c) with Wnt11 without pretreatment also displayed many brightly stained β-catenin-positive cells. (d)–(f) Higher magnification views of nontreated MSCs revealed the pattern of β-catenin fluorescence within these cells. (e), (f) High resolution of brightly stained β-catenin-positive cells shown in successive panels for β-catenin immunoreactivity only or both β-catenin and DAPI fluorescence, indicated that β-catenin protein is displayed within the cytoplasm, perinuclear region (arrow), and nucleus (arrow). Scale bar = 20 μm.