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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Mol Pharm. 2017 Apr 4;14(5):1591–1600. doi: 10.1021/acs.molpharmaceut.6b01028

Figure 6.

Figure 6

Effect of NAC and NAC-NPs on the release of NO by LPS-activated microglia. Release of nitrite (A), nitrate plus nitrite (B), and nitrate/nitrite ratio (C). BV-2 cells were co-treated with LPS (100 ng/ml) and/or 0.25 or 2 mM free NAC or NAC-NPs for 3 hours first followed by additional 72 hours of LPS treatment. The concentrations of the control-NP is equivalent to NAC-NPs providing 0.25 or 2 mM NAC. Nitrite and nitrate production was estimated in culture media using Griess reagent. Data expressed as mean ± SD (* P < 0.01).