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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Mol Pharm. 2017 Apr 4;14(5):1591–1600. doi: 10.1021/acs.molpharmaceut.6b01028

Figure 7.

Figure 7

Effect of NAC and NAC-NPs on the production of ROS by LPS-activated microglia. ROS generation was measured by DCFH-DA fluorescence intensity (A) and visualized by confocal microscopy (B). The nuclei were stained with DAPI (blue) and the ROS was detected by DCFH-DA (green). The production of superoxide was detected by hydroethidine and visualized by confocal microscopy as indicated by the converting blue fluorescent signal to red (C). BV-2 cells were pre-treated with 2 mM free NAC or NAC-NPs in the presence of 100 ng/ml LPS for 3 hours followed by additional 24 hours of LPS (100 ng/ml) treatment. Data expressed as mean ± SD (* P < 0.01).