Table 2.

PCR Cycling Conditions: Chlamydia genus specific

Cycle Conditions	First Round	Second Round
PrePCRdenaturation	94°C – 3 min	94°C – 2 min
Denaturation	94°C – 10 s	94°C – 10 s
Annealing	45°C – 10 s	45°C – 10 s
Extension	72°C – 30 s	72°C – 30 s
Number of Cycles	35	35