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. 2001 Aug 24;1:12. doi: 10.1186/1471-2334-1-12

Table 3.

PCR Cycling Conditions: HLA DRB gene

Cycle Conditions First Round
PrePCRdenaturation 96°C – 5 min
Denaturation 96°C – 1 min
Annealing 55 °C – 1 min
Extension 72°C – 1 min
Number of Cycles 40