Table 3.
Cycle Conditions | First Round |
PrePCRdenaturation | 96°C – 5 min |
Denaturation | 96°C – 1 min |
Annealing | 55 °C – 1 min |
Extension | 72°C – 1 min |
Number of Cycles | 40 |
Cycle Conditions | First Round |
PrePCRdenaturation | 96°C – 5 min |
Denaturation | 96°C – 1 min |
Annealing | 55 °C – 1 min |
Extension | 72°C – 1 min |
Number of Cycles | 40 |