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. 2017 Jul 31;8:160. doi: 10.1038/s41467-017-00233-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interactions among the subunits in the MDC hetero-tetramer. a Interactions between MdcA (domain AC, orange) and MdcC (light blue) in MDC. b Interactions between MdcC (light blue) and MdcE (green) in MDC. c Interactions between MdcA (domain AC, orange) and MdcE (green) in MDC. MdcD (cyan) makes a small contribution to this interface. d Gel filtration profiles showing that the MDC hetero-tetramer can be formed by mixing its subunits. MdcACDE: the hetero-tetramer purified from co-expressing the four subunits; MdcA+C+DE: mixture of purified MdcA, MdcC, and MdcD–MdcE subunits. e Gel filtration profiles showing that the sub-complexes of MDC are not stable, with the exception of MdcD–MdcE. f Nickel pull-down experiments showing the effects of mutations in the MDC interface on hetero-tetramer formation. Only MdcE carries a His tag. The deletion mutations abolished the complex, as no MdcA nor MdcC was pulled down, while most of the single-site mutations had essentially no effect