Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 31;8:159. doi: 10.1038/s41467-017-00188-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Stalled ribosomes in a rotated state conformation are targets for RQC. a The anisomycin sensitivity of rqt and uS10-K6/8R mutants. b Ribosome short footprints were accumulated at CGA-CGA codons. The short footprints and long footprints from the ribosome profiling data set were mapped on the reporter gene (GFP-R12-FLAG-HIS3). The reads were plotted at approximate position of ribosome A-site. c Translation of HIS3 downstream of R(CGN)12 arrest-sequence was increased in the rqt and uS10-K6/8R mutants. d Codon specificity of poly-arginine sequence to induce translation arrest and RQC. e Specificity of di-codons for accumulation of short and long footprints. f GFP-X-FLAG-HIS3 reporter assay of Top 10 di-codons in the accumulation of short footprints. g GFP-X-FLAG-HIS3 reporter assay of top 4 di-codons in the accumulation of long footprints

HHS Vulnerability Disclosure