Fig. 6.

Rqt1-bound ribosomes are the rotated form with hybrid tRNAs. a CBB-stained SDS gel and Amidoblack-stained blot of pullouts from Rqt1-FTP strains. b Particle sorting was done by 3D classification in RELION using four classes starting from 142,001 particles. This resulted in three dominant classes with rotated ribosomes (two classes in rotated state 1 and one class in rotated state 2) and one class with un-rotated (classical) state ribosomes; the highest-resolution close-to-focus data set was refined using movie processing (see “Methods” section). In total, the data set contained 22.4% classical state and 77.6% rotated state ribosomes. c Population of programmed and unprogrammed ribosomes in Rqt1-FTP and uL30-TAP pullouts