Table 4.

TNF residues in contact (d < 3.8 Å) with the three VHHs.

TNF	Residues	VHH#1	VHH#2	VHH#3
20	B
21	B			X
22	B			X
23	B			X
24	B			X
25	B			X
65	B			X
66	B			X
67	B			X
70	B			X
72	B
73	B
74	B
77	B
79	B
81	B
83	B
83	B
88	B
89	B
90	B
91	B
92	B
97	B
107	B
135	B
136	B
137	B
138	B
139	B
140	B
141	B
115	C
145	C
146	C
147	C

Shaded boxes represent residues within TNF that are in contact with residues of the indicated Nanobody.

TNF, tumor necrosis factor; VHH, variable-domain heavy-chain region.