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. 2017 Jul 31;8:1315. doi: 10.3389/fpls.2017.01315

FIGURE 6.

FIGURE 6

MeSAUR1 binding to the promoter of MeAGPS1a as analyzed DPI-ELISA. (A) Over-expression of MeSAUR1 in Escherichia coli. M: molecular markers, (1) E. coli cells harboring pCold Pros2-MeSAUR1 not induced; (2) E. coli cells harboring pCold Pros2-MeSAUR1 after 5 h of induction; (3) the purified MeSAUR1 fusion protein by E. coli cells harboring pCold Pros2-MeSAUR1 after 5 h of induction. (B) Western blot of purified MeSAUR1 fusion protein. M: Molecular markers; (1) MeSAUR1 fusion protein; (2) tagged protein ProS2. (C) MeSAUR1 interacts with the MeAGPS1a promoter by DPI-ELISA assay. MeSAUR1: double-strand biotinylated MeAGPS1a promoter DNA probe + purified MeSAUR1 fusion protein; CK1: double-strand biotinylated MeAGPS1a promoter DNA probe + tagged protein ProS2; CK2: purified water + purified MeSAUR1 fusion protein. Error bars represent the SD of four technical replicates, the significant difference is assessed by ANOVA at P < 0.01. A–C means the significant difference at p < 0.01 level.