Table 1.
Primer name | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
V1 | AAGCTTCAGCTGCCCCTAC | GTCGACTAGCAAGTTCAGATTTGG |
V2 | CATATGGCGATCAGGAAATCAAC | GTCGACTCTGAGCATGGATG |
V3 | GGATCCATGGCGATCAGGA | GTCGACTCTGAGCATGGATG |
V4 | GAATTCCAGCTGCCCCTACC | ACTAGTTAGCAAGTTCAGATTTGGAAAA |
seg5 | AAGCTTCAGCTGCCCCT | GTCGACCTAGATAATTTTTAAAATTAATAAATTTTA |
seg4 | AAGCTTCTAGTAATATGGTATTATAATTAAGG | GTCGACTAAAGTGAAGAGACG |
seg3 | AAGCTTCATGATGGTTGAAACA | GTCGACTTCTTTTTAGTAAACAAAAGA |
seg2 | AAGCTTAATACTGACGGTCCTA | GTCGACAGTGTGATTGGGG |
seg1 | AAGCTTCCGTCCCTCCAA | GTCGACTAGCAAGTTCAGATTT |
Biotin-seg2 | Biotin-AATACTGACGGTCCTAAAAATCTTAG | Biotin-AGTGTGATTGGGGCAGGG |
Biotin-AGPS1a | Biotin-CAGCTGCCCCTACCGTTAA | Biotin-TAGCAAGTTCAGATTTGGAAAAAACC |
MeSAUR1 | GGCGATCAGGAAATCAACCAA | GGACAATGTACCTGCTTCTGTT |
β-actin | CAAGGGCAACATATGCAAGC | CCTTCGTCTGGACCTTGCTG |