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. 2017 Jul 31;8:132. doi: 10.3389/fpsyt.2017.00132

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Copyright © 2017 Ito, Kawasaki, Suzuki, Takahara and Ishida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Climbing activity of A30P Parkinson’s disease (PD) model flies was dramatically decreased by sleep deprivation. (A) Schematics of sleep deprivation assay experiment. A30P PD model flies were given substances (0.25%) since eclosion. From 20 to 42 days after eclosion, sleep deprivation was repeated minutely between ZT 12 and 16. Climbing assay was performed at 45 days after eclosion. (B) Climbing activity of A30P PD model Drosophila was recovered by Kampo medicine. Each experiment was repeated for three times (n = 52 flies each). * indicates significant differences (p < 0.05).