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. 2017 Jul 31;8:890. doi: 10.3389/fimmu.2017.00890

Figure 2.

Figure 2

Inducible nitric oxide synthase (iNOS) upregulation by interferon (IFN)β in primary murine hepatocytes. (A,B) Primary murine hepatocytes were either kept as unstimulated control or stimulated with interleukin (IL)-1β/tumor necrosis factor (TNF)α (each 2 ng/ml), with IFNβ (at 250 U/ml), or with IL-1β/TNFα (each 2 ng/ml)/IFNβ (at 250 U/ml). After 8 h (A) and 16 h (B) cells and culture supernatants were harvested. (A) iNOS mRNA determined by realtime PCR was normalized to that of GAPDH and is shown as fold-induction compared with unstimulated control (mean ± SEM, n = 3; ***P < 0.001 versus unstimulated control, ##P < 0.01, ###P < 0.001). Statistical analysis, raw data were analyzed by one-way ANOVA with post hoc Bonferroni correction. (B) Nitrite production was determined using the Griess-assay (mean ± SEM, n = 3; ***P < 0.001 versus unstimulated control, ###P < 0.001). Statistical analysis, data were analyzed by one-way ANOVA with post hoc Bonferroni correction.