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. 2017 May 9;7(5):e1125. doi: 10.1038/tp.2017.85

Figure 3.

Figure 3

Pdxdc1 mRNA and protein expression in mouse brain. (a) Sample immunohistochemical (IHC) and in situ hybridization (ISH) images for Pdxdc1 protein and mRNA, respectively, from adult male mice using a visible light microscope. (b) Pdxdc1 mRNA expression in PPI-associated brain regions measured with ISH (n=7 mice) and (c) Pdxdc1 protein expression measured with IHC, in naïve male C567BL/6J mice (n=6) at 12 weeks of age. DAB (IHC): mean=0.151±0.003 (s.e.m.), 2xs.e.m. (+)=0.157 (−)=0.144. ISH: mean=53.5±1.37 (s.e.m.), 2xs.e.m. (+)=56.2 (−)=50.7. Thick dotted lines indicate whole brain averages. Thin dotted lines (+2s.e.m., −2s.e.m.) represent the whole brain confidence interval (at 2xs.e.m.) for the mean. (d) PPI regions linear regression for DAB and ISH: r2=0.609, SE of the estimate of residuals=0.032. 95% confidence interval (CI): Slope 0.00099 to 0.00183. Correlation analysis Spearman’s rho=0.706, 95% CI: 0.465 to 0.849, two-tailed P<0.0001 (32 x,y pairs). (e) Linear regression for all sampled regions. r2=0.5765, 95% CI: 0.0486 to 0.0836 (105 x,y pairs). Correlation analysis: Spearman’s rho=0.7003, 95% CI: 0.584 to 0.789, two-tailed P<0.0001. Aud Ctx, 1° & 2° auditory ctx; BLP, basolateral amygdala; posterior; BLA.p, basolateral amygdale; anterior; CA1, field CA1 of hippocampus; CA2, field CA2 of hippocampus; CA3, field CA3 of hippocampus; CIC, central nucleus; inferior colliculus; Cing ctx mid, 1° & 2° cingulate ctx areas; Cing PFC, cingulate ctx area1; CPu, caudate putamen; DG, dentate gyrus; DRN, dorsal raphe nucleus; FrA Ctx, frontal association ctx; GP, globus pallidus; LVO ctx, lateral/ventral orbital ctx; MO Ctx, medial orbital ctx; MS, medial septal nucleus; MDL/C/M, mediodorsal thalamic nuclei; NAcC, nucleus accumbens core; NAcS, nucleus accumbens shell; P/MnR, paramedian and medial raphe nuclei; Pn, pontine nucleus; PnC, caudal pontine reticular nucleus; PPI, prepulse inhibition; PTg, pedunculotegmental nucleus; SNC, substantia nigra pars compacta; SNR, substantia nigra pars reticulata; Sp5O, spinal trigeminal tract; Sup Coll, superior colliculus; VCA, anterior ventral cochlear nucleus; Ve muc, vestibular nucleus; VP, ventral pallidum.