Figure 4.

PKA-mediated BR-C phosphorylation did not influence its nuclear import. A, the two mutant constructs pSL1180-BR-C S186A (A, blocks phosphorylation) and pSL1180-BR-C S186E (E, mimics phosphorylation) were transfected into BmN4-SID1 cells. Two days after transfection the localization of the BR-C protein was analyzed by immunostaining and confocal microscopy. Intact silkworm BR-C was used as a control. B, 2 days after transfection with the overexpression vector pSL1180-BR-C, the BmN4-SID1 cells were treated with PKA inhibitors for 4 h, and the fluorescence signals were detected with confocal microscopy. DMSO treatment was used as a control (Con). KT, KT5720. Wheat germ agglutinin (WGA) is used in nuclear membrane staining.