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. 2017 Jun 5;292(30):12460–12470. doi: 10.1074/jbc.M117.775130

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — PKA-mediated BR-C phosphorylation and the cAMP/PKA pathway were attenuated by 20E. A, fat bodies were isolated from wandering silkworm individuals and then treated ex vivo with 2 μm 20E for 12 h. The change in BR-C phosphorylation level after 20E treatment was examined. B, after being transfected with pSL1180-BR-C for 24 h, the BmE cells were treated with 2 μm 20E and then collected to analyze BR-C phosphorylation level. C, BmE cells were treated with 2 μm 20E, and the intracellular cAMP was measured. D, effect of 2 μm 20E on PKA-C phosphorylation in BmE cells for various time periods. E, effect of 2 μm 20E on CREB protein phosphorylation in BmE cells. F, effect of 20E on CREB protein phosphorylation in ex vivo cultured fat bodies from the wandering silkworm individuals. DMSO treatment was used as a control. Con, control.