Figure 1.

Gene expression analysis of biopsies of healthy skin and cSCC tumor samples. A, example of tissue sections of healthy mouse skin (S) and DMBA/TPA-induced cSCC tumors (T) used for the transcriptomic analysis. Pictures correspond to hematoxylin/eosin-counterstained formalin-fixed paraffin-embedded sections. B, RNAs extracted from three individual samples of healthy skin treated with acetone only (S1–S3) and three DMBA/TPA-generated cSCC tumors (T1–T3) (see A) were labeled with Cy3 and hybridized with mouse gene expression 8 × 60,000 v1 microarrays from Agilent as described under “Experimental procedures.” The graph shows the heat map comparing the normalized log₂ of gene intensity signal in the different conditions. The following threshold values were used to define the set of up- and down-regulated genes: average expression >7.0, absolute log FC >1.0, adjusted p value <0.05.